Mouse Fibronectin ELISA Kit (RMEK-0153738)
Cat. No.: RMEK-0153738
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse Fibronectin ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fibronectin protein in cell culture supernatant, plasma, serum, and urine. The technology. Quantitate Mouse Fibronectin with 251 pg/mL sensitivity.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-5-2.3%; Inter-Assay-Serum-3-4.4% |
| Sensitivity | 251 pg/mL |
| Range | 0.63 ng/mL - 40 ng/mL |
| Sample Type | Cell culture supernatant, Plasma, Serum, Urine |
| Recovery | Cell culture supernatant-102-101% - 103%; Urine-107-104% - 113%; Serum-119-117% - 123%; Hep Plasma-108-106% - 109%; EDTA Plasma-107-103% - 112%; Cit plasma-120-119% - 122% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 251 pg/mL; Range: 0.63 ng/mL - 40 ng/mL; Sample type: Cell culture supernatant, Plasma, Serum, Urine; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | FN |
|---|---|
| UniProt ID | P02751 |
| Function | Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. |
| Development Stage | Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. |
| Post-transcriptional Modifications | Sulfated.It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).Phosphorylated by FAM20C in the extracellular medium.Proteolytic processing produces the C-terminal NC1 peptide, anastellin. |
| Involvement in Disease | Glomerulopathy with fibronectin deposits 2 |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
