Mouse HDAC8 ELISA Kit (RMEK-0153775)
Cat. No.: RMEK-0153775
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse HDAC8 ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse HDAC8 protein in mouse cell and tissue extract samples. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-5.9%; Inter-Assay-Extract-0-0% |
| Sensitivity | 13 pg/mL |
| Range | 140.63 pg/mL - 9000 pg/mL |
| Sample Type | Cell Lysate |
| Recovery | Cell Lysate-104-92% - 118% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 13 pg/mL; Range: 140.63 pg/mL - 9000 pg/mL; Sample type: Cell Lysate; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | HDAC8 |
|---|---|
| UniProt ID | Q9BY41 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. |
| Cellular Localization | Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation. |
| Post-transcriptional Modifications | Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
