Mouse ICAM 1 ELISA Kit (RMEK-0153786)
Cat. No.: RMEK-0153786
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse ICAM 1 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ICAM 1 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue culture media. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-4.6%; Inter-Assay-Serum-3-4% |
| Sensitivity | 28.15 pg/mL |
| Range | 62.5 pg/mL - 4000 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Culture Media |
| Recovery | Cell culture supernatant-113-107% - 115%; Serum-96-81% - 111%; Tissue Extracts-91-82% - 99%; Hep Plasma-91-84% - 100%; EDTA Plasma-99-90% - 104%; Cit plasma-102-86% - 113% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 28.15 pg/mL; Range: 62.5 pg/mL - 4000 pg/mL; Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Culture Media; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | ICAM1 |
|---|---|
| UniProt ID | P05362 |
| Biomarker of SCs/CSCs | Hepatic Cancer |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. |
| Cellular Localization | Membrane. |
| Post-transcriptional Modifications | Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
