Mouse IL-11 ELISA Kit (RMEK-0153790)
Cat. No.: RMEK-0153790
Category: ELISA Kits
INQUIRY
1 x 96 tests
IL-11 Mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-11 in plasma and cell culture supernatants. (Mouse IL-11 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for mouse IL-11 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-11 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This assay employs an antibody specific for mouse IL-11 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-11 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | Multiple steps standard assay |
| Assay Type | Sandwich (quantitative) |
| Sensitivity | Sensitivity: 1 ng/mL |
| Range | 1.85 ng/mL - 450 ng/mL |
| Sample Type | Cell culture supernatant, Plasma |
| Recovery | Cell culture supernatant-77.35-70% - 87%; Plasma-117.3-95% - 113% |
| Key Features | Sensitivity: 1 ng/mL; Range: 1.85 ng/mL - 450 ng/mL; Sample type: Cell culture supernatant, Plasma; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | IL11 |
|---|---|
| UniProt ID | P20809 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. |
| Cellular Localization | Secreted. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
