Mouse IL-6 R alpha ELISA Kit (RMEK-0153791)
Cat. No.: RMEK-0153791
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse IL-6 R alpha ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-6 R alpha protein in cell culture supernatant, serum, urine, and plasma. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Mouse Serum-5-1.8%; Inter-Assay-Mouse Serum-3-12% |
| Sensitivity | 12.6 pg/mL |
| Range | 31.3 pg/mL - 2000 pg/mL |
| Sample Type | Cell culture supernatant, Plasma, Serum, Urine |
| Recovery | Urine-85-80% - 89%; Serum-105-103% - 106%; Cell culture media-88-84% - 93%; Hep Plasma-103-101% - 105%; EDTA Plasma-103-102% - 103%; Cit plasma-104-100% - 107% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 12.6 pg/mL; Range: 31.3 pg/mL - 2000 pg/mL; Sample type: Cell culture supernatant, Plasma, Serum, Urine; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | IL6Ralpha |
|---|---|
| UniProt ID | P08887 |
| Function | Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity. |
| Cellular Localization | Secreted and Basolateral cell membrane. |
| Domain | The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. |
| Post-transcriptional Modifications | A short soluble form may also be released from the membrane by proteolysis. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
