Mouse KLF4 ELISA Kit (RMEK-0153820)
Cat. No.: RMEK-0153820
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse KLF4 ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse KLF4 protein in human cell extract samples. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-2.5%; Inter-Assay-Extract-3-5.1% |
| Sensitivity | 26 pg/mL |
| Range | 96.88 pg/mL - 6200 pg/mL |
| Sample Type | Cell Lysate |
| Recovery | Cell Lysate-101-92% - 106% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 26 pg/mL; Range: 96.88 pg/mL - 6200 pg/mL; Sample type: Cell Lysate; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | KLF4 |
|---|---|
| UniProt ID | O43474 |
| Biomarker of SCs/CSCs | Bladder Cancer |
| Function | Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. |
| Cellular Localization | Nucleus. |
| Domain | the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
