Mouse Leptin ELISA Kit (RMEK-0153828)
Cat. No.: RMEK-0153828
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse Leptin ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Leptin protein in cell culture supernatant, cit plasma, serum, and urine. The technology. Quantitate Mouse Leptin with 33. 6 pg/mL sensitivity. _x000D_
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-5-5.9%; Inter-Assay-Serum-3-11.5% |
| Sensitivity | 33.6 pg/mL |
| Range | 93.75 pg/mL - 6000 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, Serum, Urine |
| Recovery | Urine-80-77% - 82%; Serum-111-110% - 114%; Cell culture media-108-107% - 108%; Hep Plasma-106-94% - 116%; Cit plasma-104-100% - 111%; serum free media-104-103% - 105% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 33.6 pg/mL; Range: 93.75 pg/mL - 6000 pg/mL; Sample type: Cell culture supernatant, Cit plasma, Serum, Urine; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | LEP |
|---|---|
| UniProt ID | P41159 |
| Function | May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. |
| Cellular Localization | Secreted. |
| Involvement in Disease | Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
