Mouse MAP2 ELISA Kit (RMEK-0153842)
Cat. No.: RMEK-0153842
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse MAP2 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MAP2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-5.8%; Inter-Assay-Serum-3-2.5% |
| Sensitivity | 43.31 pg/mL |
| Range | 156.25 pg/mL - 10000 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Cell culture supernatant-93-90% - 99%; Serum-84-82% - 87%; Tissue Extracts-81-80% - 83%; Hep Plasma-91-93% - 101%; EDTA Plasma-84-82% - 89%; Cit plasma-84-81% - 86% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 43.31 pg/mL; Range: 156.25 pg/mL - 10000 pg/mL; Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | MAP2 |
|---|---|
| UniProt ID | P11137 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Post-transcriptional Modifications | Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
