Mouse Nanog ELISA Kit (RMEK-0153857)
Cat. No.: RMEK-0153857
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse Nanog ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Nanog protein in cell culture extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-cell extract-8-7.5% |
| Sensitivity | 54.2 pg/mL |
| Range | 0.23 ng/mL - 15 ng/mL |
| Sample Type | Cell culture extracts |
| Recovery | Cell culture extracts-99-66% - 143% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 54.2 pg/mL; Range: 0.23 ng/mL - 15 ng/mL; Sample type: Cell culture extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | Nanog |
|---|---|
| UniProt ID | Q9H9S0 |
| Biomarker of SCs/CSCs | Luminal A Breast Cancer |
| Function | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation. |
| Cellular Localization | Nucleus. |
| Development Stage | Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
