Mouse NSE / Neuron-specific Enolase ELISA Kit (RMEK-0153864)
Cat. No.: RMEK-0153864
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse NSE / Neuron-specific Enolase ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NSE / Neuron-specific Enolase protein in tissue extracts, cell culture supernatant, and cell culture extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Mouse brain-5-7.1%; Inter-Assay-Mouse brain-3-4.2% |
| Sensitivity | 50.8 pg/mL |
| Range | 312.5 pg/mL - 60000 pg/mL |
| Sample Type | Cell culture extracts, Cell culture supernatant, Tissue Extracts |
| Recovery | Cell culture extracts-101-97% - 107%; Tissue Extracts-115-113% - 118%; Cell culture media-89-92% - 98% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 50.8 pg/mL; Range: 312.5 pg/mL - 60000 pg/mL; Sample type: Cell culture extracts, Cell culture supernatant, Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | ENO2 |
|---|---|
| UniProt ID | P09104 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. |
| Cellular Localization | Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. |
| Development Stage | During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
