Mouse RGMA ELISA Kit (RMEK-0153902)
Cat. No.: RMEK-0153902
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse RGMA ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RGMA protein in cit plasma, edta plasma, hep plasma, and serum. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-7%; Inter-Assay-Serum-3-8.1% |
| Sensitivity | 90 pg/mL |
| Range | 0.31 ng/mL - 20 ng/mL |
| Sample Type | Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Serum-110-105% - 115%; Hep Plasma-108-105% - 114%; EDTA Plasma-112-105% - 121%; Cit plasma-115-113% - 120% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 90 pg/mL; Range: 0.31 ng/mL - 20 ng/mL; Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | RGMA |
|---|---|
| UniProt ID | Q96B86 |
| Biomarker of SCs/CSCs | Triple Negative Breast Cancer |
| Function | Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8. |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. |
| Post-transcriptional Modifications | Autocatalytically cleaved at low pH; the two chains remain linked via two disulfide bonds. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
