Mouse SCF ELISA Kit (RMEK-0153912)
Cat. No.: RMEK-0153912
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse SCF ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SCF protein in cell culture supernatant, cit plasma, and serum. The technology. Quantitate Mouse SCF with 1. 11 pg/mL sensitivity. _x000D_
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Overall-8-2.89%; Inter-Assay-Overall-3-5.09% |
| Sensitivity | 1.11 pg/mL |
| Range | 15.63 pg/mL - 1000 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, Serum |
| Recovery | Serum-101.6-100.9% - 102.4%; Cell culture media-101.5-99.8% - 104%; Cit plasma-103.5-102.5% - 105.5% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 1.11 pg/mL; Range: 15.63 pg/mL - 1000 pg/mL; Sample type: Cell culture supernatant, Cit plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | SCF |
|---|---|
| UniProt ID | P21583 |
| Function | Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. |
| Cellular Localization | Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. |
| Development Stage | Acts in the early stages of hematopoiesis. |
| Post-transcriptional Modifications | A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.A soluble form exists as a cleavage product of the extracellular domain. |
| Involvement in Disease | Hyperpigmentation with or without hypopigmentation, familial progressiveDeafness, congenital, unilateral or asymmetric |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
