Mouse sTNF RI ELISA Kit(TNFRSF1A) (RMEK-0153960)
Cat. No.: RMEK-0153960
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse sTNF RI ELISA Kit (TNFRSF1A) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of sTNF RI (TNFRSF1A) protein in cell culture supernatant, cit plasma, edta plasma, serum, and urine. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-5-1.5%; Inter-Assay-Serum-3-13.8% |
| Sensitivity | 1.2 pg/mL |
| Range | 7.81 pg/mL - 500 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Urine |
| Recovery | Urine-103-101% - 109%; Serum-113-107% - 118%; Cell culture media-95-94% - 95%; EDTA Plasma-102-97% - 109%; Cit plasma-106-97% - 112% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 1.2 pg/mL; Range: 7.81 pg/mL - 500 pg/mL; Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Urine; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | sTNF RI |
|---|---|
| Function | Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. |
| Cellular Localization | Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted. |
| Domain | The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP. |
| Post-transcriptional Modifications | The soluble form is produced from the membrane form by proteolytic processing. |
| Involvement in Disease | Familial hibernian feverMultiple sclerosis 5 |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
