Mouse VCAM1 ELISA Kit (RMEK-0154019)
Cat. No.: RMEK-0154019
Category: ELISA Kits
INQUIRY
1 x 96 tests
VCAM1 Mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse VCAM1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for Mouse VCAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and VCAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse VCAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VCAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | Multiple steps standard assay |
| Assay Type | Sandwich (quantitative) |
| Sensitivity | Sensitivity: 1.5 pg/mL |
| Range | 2.05 pg/mL - 500 pg/mL |
| Sample Type | Cell culture supernatant, Plasma, Serum |
| Recovery | Cell culture supernatant-97.36-84% - 107%; Serum-96.43-82% - 105%; Plasma-94.64-81% - 104% |
| Key Features | Sensitivity: 1.5 pg/mL; Range: 2.05 pg/mL - 500 pg/mL; Sample type: Cell culture supernatant, Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | VCAM1 |
|---|---|
| UniProt ID | P19320 |
| Biomarker of SCs/CSCs | Small Cell Lung Cancer |
| Function | Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. |
| Cellular Localization | Membrane. |
| Domain | Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. |
| Post-transcriptional Modifications | Sialoglycoprotein. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
