Mouse VCAM1 ELISA Kit(CD106) (RMEK-0154020)
Cat. No.: RMEK-0154020
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse VCAM1 ELISA Kit (CD106) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VCAM1 (CD106) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Overall-8-4.3%; Inter-Assay-Overall-3-5.6% |
| Sensitivity | 5.59 pg/mL |
| Range | 46.9 pg/mL - 3000 pg/mL |
| Sample Type | Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts |
| Recovery | Serum-107-104% - 109%; Cell culture media-87-86% - 88%; Hep Plasma-108-107% - 108%; EDTA Plasma-109-106% - 111%; Cit plasma-111-109% - 113% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 5.59 pg/mL; Range: 46.9 pg/mL - 3000 pg/mL; Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | VCAM1 |
|---|---|
| UniProt ID | P19320 |
| Biomarker of SCs/CSCs | Small Cell Lung Cancer |
| Function | Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. |
| Cellular Localization | Membrane. |
| Domain | Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. |
| Post-transcriptional Modifications | Sialoglycoprotein. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
