OP9 (RMCL-00260295)
Cat. No.: RMCL-00260295
Category: Cell Lines
INQUIRY
1 vial
OP9 is a macrophage-derived embryonic stem cell line that was isolated from the stroma of a mouse embryo. This cell line was deposited by T Nakano and can be used in stem cell research.
Product Features
| BSL | BSL-1 |
|---|---|
| Product Format | Frozen |
| Applications | Immunology; Stem cell research |
| Specific Applications | OP9 cells can be used to coculture mouse embryonic stem cells (ES cells) to induce the differentiation of embryonic stem (ES) cells into blood cells of erythroid, myeloid, and B cell lineages. Cocultivation with OP9 does not require exogenous growth factors or complex embryoid structures. This system will facilitate the study of molecular mechanisms involved in development and differentiation of hematopoietic cells. |
| Product Type | Animal cells |
| Strain | (C57BL/6 x C3H)F2 -op/op |
| Organism | Mus musculus, mouse |
| Tissue | Bone; Marrow; Stroma |
| Morphology | Fibroblast-like |
| Cell Type | Embryonic Stem Cell |
QC
| Mycoplasma Contamination | Not detected |
|---|
Doner Information
| Age | Embryo |
|---|
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Reagents for Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Growth Properties | Adherent |
| Temperature | 37°C |
| Atmosphere | 95% Air, 5% CO2 |
| Handling procedure | To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes). Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions. It is recommended that the cryoprotective agent be removed immediately. Centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes. Discard the supernatant and resuspend the cell pellet in an appropriate amount of fresh growth medium. Transfer the cells to an appropriate size vessel. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7. 0 to 7. 6). Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet. |
| Population Doubling Time | Approximately 26 hrs |
For research use only. Not for clinical use.
