Primary Bone Marrow CD34+ Cells, Normal, Human (RMCL-00260333)
Cat. No.: RMCL-00260333
Category: Primary cell
INQUIRY
1 vial
Primary Bone Marrow CD34+ Cells, Normal, Human are hematopoietic pluripotent stem cells isolated by immunomagnetic positive selection. CD34+ bone marrow-derived and CD34+ cord blood-derived cells may be used to support physiologically relevant research in regenerative medicine, transplant studies, stem cell biology, hematology, and oncology.
Product Features
| BSL | BSL-1 |
|---|---|
| Product Format | Frozen |
| Applications | 3D cell culture; Immunology; Stem cell research; Toxicology; Immuno-oncology; Cell and gene therapy (CGT) development |
| Specific Applications | Applications for use include the study of stem cell biology, hematology, transplantation, and oncology. |
| Product Type | Human cells |
| Organism | Homo sapiens, human |
| Tissue | Bone |
| Morphology | Spherical |
| Cell Type | Hematopoietic Stem |
| Morphology Note | Variable after culturing |
| Disease | Normal |
| Specific Staining | Positive for CD34 and CD45 |
QC
| Virus Testing | Human T-lymphotropic virus 2 (HTLV-2): Not detected Human immunodeficiency virus 1 (HIV-1): Not detected West Nile virus (WNV): Not detected Trypanasoma cruzi: Not detected Hepatitis C virus (HCV): Not detected Hepatitis B virus (HBV): Not detected Human T-lymphotropic virus 1 (HTLV-1): Not detected Human immunodeficiency virus 2 (HIV-2): Not detected |
|---|
Doner Information
| Gender | Lot-specific |
|---|---|
| Ethnicity | Lot-specific |
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Growth Properties | Suspension, variable after culturing |
| Handling procedure | Rapidly thaw cryovial in a 37°C water bath. Remove the vial before all the ice is thawed. Decontaminate the cryovial with ethanol and move it to a biosafety cabinet. While on ice, transfer contents of the vial to a 15 mL conical tube. Rinse the cryovial with 1 mL of ice-cold thawing media (Hank's Balanced Salt Solution without Ca2+ or Mg2+, supplemented with 10% Fetal Bovine Serum and slowly transfer to the cells in the conical tube. Slowly pipet 10X the vial volume of thawing media into the conical tube Take a sample for counting and viability assessment. Centrifuge the remaining contents of the vial at 4°C at 300 x g for 5 minutes to pellet cells. Aspirate the wash leaving a few mL behind. Re-suspend cells in the media of choice. |
For research use only. Not for clinical use.
