Primary Gingival Fibroblast; Normal, Human, Adult (HGF) (RMCL-00260311)
Cat. No.: RMCL-00260311
Category: Primary cell
INQUIRY
1 vial
Primary gingival fibroblast was isolated from the gingiva. The cell has applications in human gingival fibroblasts (hGF) and could potentially be an alternative source of mesenchymal stem cells (MSC) for regenerative medicine studies as they share similar morphology, CD markers, and differentiation lineage.
Product Features
| BSL | BSL-1 |
|---|---|
| Product Format | Frozen |
| Applications | 3D cell culture; Stem cell research |
| Specific Applications | Human gingival fibroblasts (hGF) could potentially be an alternative source of mesenchymal stem cells (MSC) for regenerative medicine studies as they share similar morphology, CD markers, and differentiation lineage. |
| Product Type | Human cells |
| Organism | Homo sapiens, human |
| Tissue | Gingiva |
| Morphology | Spindle-shaped |
| Cell Type | Fibroblast |
| Morphology Note | Cells are bipolar and refractile |
| Specific Staining | TE-7 (+), Pan-Cytokeratin (-) |
QC
| Bacterial & Fungal Testing | Not detected |
|---|---|
| Mycoplasma Contamination | Not detected |
| Virus Testing | Human immunodeficiency virus 1 (HIV-1): Not detected Hepatitis C virus (HCV): Not detected Hepatitis B virus (HBV): Not detected Human immunodeficiency virus 2 (HIV-2): Not detected |
Doner Information
| Gender | Female |
|---|---|
| Age | 60 years |
| Ethnicity | White |
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Growth Properties | Adherent |
| Temperature | 37°C |
| Atmosphere | 95% Air, 5% CO2 |
| Handling procedure | To ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes). Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions. Transfer the vial contents to a centrifuge tube containing 9. 0 mL complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes. Discard supernatant and resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7. 0 to 7. 6). pH (7. 0 to 7. 6). Refer to the batch-specific information provided on the Certificate of Analysis for the total number of viable cells recovered from this lot of this product |
| Population Doubling Capacity | 15 PDL |
For research use only. Not for clinical use.
