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Primary Subcutaneous Pre-adipocytes; Normal, Human

Primary Subcutaneous Pre-adipocytes; Normal, Human (RMCL-00260234)

Cat. No.: RMCL-00260234

Category: Cell Lines

INQUIRY 1 vial
Normal Human Pre-Adipocytes were isolated from the adipose tissue of a donor and are derived from de-differentiated mature adipocytes by ceiling culture and providing an ideal culture model for the study of diabetes, obesity, metabolism, insulin sensitivity, and adipose biology.

Product Features

BSL BSL-1
Product Format Frozen
Applications Stem cell research
Specific Applications Adult stem cell differentiation, regenerative medicine, cell therapy, tissue engineering, creation of iPS cell lines
Functional Test Each lot is tested to ensure in vitro differentiation to mature adipocytes.
Product Type Human cells
Organism Homo sapiens, human
Tissue Adipose tissue
Morphology Spindle-shaped, fibroblast-like
Specific Staining Positive for CD29, CD44, CD73, CD90, CD105, and CD166.(greater than 95% of the cell population expresses these markers by flow cytometry) Negative for CD14, CD31, CD34, and CD45 (less than 2% of cell population expresses these markers by flow cyt)

QC

Bacterial & Fungal Testing Not detected
Mycoplasma Contamination Not detected
Virus Testing Human immunodeficiency virus 1 (HIV-1): Not detected
Hepatitis C virus (HCV): Not detected
Hepatitis B virus (HBV): Not detected
Human immunodeficiency virus 2 (HIV-2): Not detected

Doner Information

Gender Lot-specific
Age Adult
Ethnicity Lot-specific

Storage & Shipping

Storage Conditions Vapor phase of liquid nitrogen
Unpacking and Storage Instructions Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
Growth Properties Adherent
Handling procedure Refer to the batch specific information provided on the last page of the product information sheet for the total number of viable cells recovered from this lot of this product
Population Doubling Capacity ≥ 10 in complete growth medium and support differentiation

For research use only. Not for clinical use.