Rabbit Octamer Binding Transcription Factor 4 ELISA Kit (RMEK-0154867)
Cat. No.: RMEK-0154867
Category: ELISA Kits
INQUIRY
1 x 96 tests
This ELISA kit is a 1. 5 hour solid-phase ELISA designed for the quantitative determination of the targets. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Features
| Species Reactivity | Rabbit |
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| Assay Time | 1.5 h |
Target Information
| Target Symbol | OBTF4 |
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| UniProt ID | Q01860 |
| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Cellular Localization | Cytoplasm. Nucleus. Expressed in a diffuse and slightly punctuate pattern. Colocalizes with MAPK8 and MAPK9 in the nucleus. |
| Development Stage | Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation. |
| Domain | The POU-specific domain mediates interaction with PKM. The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. |
| Post-transcriptional Modifications | Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
