Rat L-Selectin ELISA Kit (RMEK-0155325)
Cat. No.: RMEK-0155325
Category: ELISA Kits
INQUIRY
1 x 96 tests
Rat L-Selectin ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of L-Selectin protein in cell culture supernatant, cit plasma, edta plasma, and serum. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Rat |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Plasma-8-6.5%; Inter-Assay-Plasma-3-10.9% |
| Sensitivity | 0.77 ng/mL |
| Range | 1.6 ng/mL - 100 ng/mL |
| Sample Type | Cell culture supernatant, Cit plasma, EDTA Plasma, Serum |
| Recovery | Cell culture supernatant-120-119% - 120%; Serum-90-86% - 95%; EDTA Plasma-88-82% - 95%; Cit plasma-89-84% - 96% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 0.77 ng/mL; Range: 1.6 ng/mL - 100 ng/mL; Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Rat |
Target Information
| Target Symbol | SELL |
|---|---|
| UniProt ID | P14151 |
| Biomarker of SCs/CSCs | Hepatic Cancer |
| Function | Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. |
| Cellular Localization | Cell Membrane; single-pass type I membrane protein. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
