Rat TNF alpha ELISA Kit Set (without plates) (RMEK-0155458)
Cat. No.: RMEK-0155458
Category: ELISA Kits
INQUIRY
5 x 96 tests
TNFα Rat in vitro ELISA Set is designed for the quantitative measurement of Rat TNFα in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The rat TNF alpha ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure rat TNF alpha in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant Rat TNF alpha Range: 31. 25 pg/mL - 1000 pg/mL Sensitivity < 15 pg/mL
The rat TNF alpha ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure rat TNF alpha in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant Rat TNF alpha Range: 31. 25 pg/mL - 1000 pg/mL Sensitivity < 15 pg/mL
Product Features
| Assay Duration | Multiple steps standard assay |
|---|---|
| Assay Type | Sandwich (quantitative) |
| Range | 31.25 pg/mL - 1000 pg/mL |
Target Information
| Target Symbol | TNF alpha |
|---|---|
| UniProt ID | P01375 |
| Function | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. |
| Cellular Localization | Secreted and Cell membrane. |
| Post-transcriptional Modifications | The soluble form derives from the membrane form by proteolytic processing.The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. |
| Involvement in Disease | Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
