Recombinant Human BRG1 Protein (denatured) (RMPP-00231029)
Cat. No.: RMPP-00231029
Category: Kinases
Research Area: Signal Transduction
INQUIRY
100 μg
Customer Size
Product Features
| Source | Baculovirus infected Sf9 cells |
|---|---|
| Purity | > 90% SDS-PAGE. Affinity purified. |
| Nature | Recombinant |
| Animal Free | No |
| Form | Liquid |
| Applications | WB; SDS-PAGE |
| Key Features | Expression system: Baculovirus infected Sf9 cells; Purity: > 90% SDS-PAGE; Suitable for: WB, SDS-PAGE |
Protein Information
| UniProt ID | P34925 |
|---|---|
| Molecular Weight | 66 kDa including tags |
| Sequence | MKRIELDDSISASSSSQGLSQPSTQTTQYLRADTPNNATPITSSLGYPTL RIEKNDLRSVTLLEAKGKVKDIAISRERITLKDVLQEGTFGRIFHGILID EKDPNKEKQAFVKTVKDQASEIQVTMMLTESCKLRGLHHRNLLPITHVCI EEGEKPMVILPYMNWGNLKLFLRQCKLVEANNPQAISQQDLVHMAIQIAC GMSYLARREVIHKDLAARNCVIDDTLQVKITDNALSRDLFPMDYHCLGDN ENRPVRWMALESLVNNEFSSASDVWAFGVTLWELMTLGQTPYVDIDPFEM AAYLKDGYRIAQPINCPDELFAVMACCWALDPEERPKFQQLVQCLTEFHA ALGAYV |
| Sequence Similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 1 protein kinase domain. Contains 1 WIF domain. |
| Protein Length | Protein fragment |
| Cellular Localization | Membrane. Nucleus. Cytoplasm. In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus. |
| Tissue Specificity | Observed in all the tissues examined. |
| Domain | The extracellular WIF domain is responsible for Wnt binding. |
| Function | May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development. |
| Post-translational Modifications | Proteolytically cleaved, in part by presenilin, in response to WNT3 stimulation. Cleavage occurs during neuronal differentiation. |
Storage & Shipping
| Shipping and Storage | Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. pH: 7.50Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride |
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For research use only. Not for clinical use.
