Recombinant Human sIL-6 Receptor α (CHO derived) (RMPP-00231341)
Cat. No.: RMPP-00231341
Category: Growth Factors & Cytokines
INQUIRY
5 μg
20 μg
IL-6 mediates its biological effects thro μgh the type I IL-6 receptor system that consists of two chains, IL-6Rα and gp130. While the IL-6Rα chain is the binding component specific to IL-6, the gp130 chain only transmits signals of IL-6 when bound to IL-6Rα. The gp130 can also transmit signals from LIF, OSM, CNTF, IL-11 and CT-1 in conjunction with other receptor subunits. The low-affinity binding site for IL-6 is composed of IL-6Rα alone. IL-6Rα is expressed in a wide range of cells, including T cells, fibroblasts and macrophages. Soluble IL-6Rα, which consists of only the extracellular domain of the IL-6Rα chain, acts as an agonist of IL-6 activity at low concentrations. PeproTech's CHO cell-derived Recombinant Human sIL-6 Receptor α is a 37.9 kDa glycoprotein corresponding to 339 amino acid residues of the extracellular domain of IL-6Rα. As a result of glycosylation, Recombinant Human sIL-6 Receptor α migrates with an apparent molecular mass of approximately 57-70 kDa by SDS-PAGE gel, under reducing conditions.
Product Features
| Source | CHO cells |
|---|---|
| Purity | ≥ 95% by SDS-PAGE gel and HPLC analyses. |
| Nature | Recombinant |
| Endotoxin Level | < 1 Eu/μg |
| Cross Reactivity | Human |
Protein Information
| UniProt ID | P08887 |
|---|---|
| Molecular Weight | 37.9 kDa |
| Sequence Similarities | Belongs to the type I cytokine receptor family. Type 3 subfamily. Contains 1 fibronectin type-III domain. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. |
| Protein Length | Protein fragment |
| Cellular Localization | Secreted and Basolateral cell membrane. |
| Tissue Specificity | Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum. |
| Domain | The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. |
| Function | Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity. |
| Post-translational Modifications | A short soluble form may also be released from the membrane by proteolysis. |
Storage & Shipping
| Shipping and Storage | Shipped on Dry Ice. |
|---|
For research use only. Not for clinical use.
