Recombinant Human Sp7 / Osterix Protein (RMPP-00230723)
Cat. No.: RMPP-00230723
Category: Recombinant Protein
Research Area: Epigenetics and Nuclear Signaling
INQUIRY
10 μg
Customer Size
Product Features
| Source | Baculovirus infected insect cells |
|---|---|
| Purity | > 80% SDS-PAGE. Affinity purified. |
| Nature | Recombinant |
| Animal Free | No |
| Tags | DDDDK tag N-Terminus |
| Form | Liquid |
| Applications | SDS-PAGE; Functional Studies |
| Key Features | Expression system: Baculovirus infected insect cells; Purity: > 80% SDS-PAGE; Active: Yes; Tags: DDDDK tag N-Terminus; Suitable for: SDS-PAGE, Functional Studies |
Protein Information
| UniProt ID | Q9HAU4 |
|---|---|
| Molecular Weight | 72 kDa including tags |
| Sequence Similarities | Contains 1 C2 domain. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Contains 3 WW domains. |
| Protein Length | Protein fragment |
| Cellular Localization | Nucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts. |
| Tissue Specificity | Widely expressed. |
| Domain | The second and third WW domains are responsible for interaction with the PY-motif of R-SMAD (SMAD1, SMAD2 and SMAD3).The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain. |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. |
| Post-translational Modifications | Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1. |
Storage & Shipping
| Shipping and Storage | Shipped on Dry Ice. Store at -80°C. pH: 8.00Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride102 µg/ml FLAG peptide This product is an active protein and may elicit a biological response in vivo, handle with caution. |
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For research use only. Not for clinical use.
