Selecting and Cloning Medium (RMMS-00560041)
Cat. No.: RMMS-00560041
Category: Specialized Medium
Research Area: Antibody Development, Cell Line Development, Drug Discovery and Toxicity Testing, Hybridoma Generation
INQUIRY
45 mL
Semi-solid methylcellulose-based medium for selecting and cloning cells
Product Features
| Overview | This product is a base methylcellulose medium that requires the addition of a 2X concentrate of the user’s own liquid medium to create a custom semi-solid cloning medium. This allows the user to adapt an existing liquid cloning method to a customized semi-solid cloning method. FLEX is recommended for selection and cloning of suspension-adapted mammalian cells, including CHO cells and hybridomas. The medium is chemically defined, animal component-free, and protein-free. It does not contain L-glutamine, selection agents, or phenol red. FLEX is compatible with a wide range of selection systems, including dihydrofolate reductase (DHFR), glutamine synthetase (GS), and hypoxanthine, aminopterin, thymidine (HAT). Benefits of semi-solid cloning: Individual clones and their progeny remain localized together in a semi-solid matrix as they grow to form distinct colonies. This prevents the loss of rare, high-producing clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium, and facilitates the isolation of a diverse set of clones with a wide range of growth rates and productivities to be obtained for downstream screening. Colonies obtained from semi-solid medium have a high probability of monoclonality, allowing clonal cell lines to be generated in less time and using fewer resources than with limiting dilution cloning. Colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid growth medium for screening and expansion. |
|---|---|
| Species | Mouse, Other |
| Cell Type | CHO Cells, HEK-293, Hybridomas, Other |
| Applications | Cell Culture, Semi-Solid Cloning |
| Formulation | Animal Component-Free, Chemically Defined, Protein-Free, Serum-Free |
Storage & Shipping
| Storage | -20°C or colder |
|---|---|
| Shipping | Shipped on gel packs. |
For research use only. Not for clinical use.
