SF Expansion Supplement Kit (Human) (RMMS-00560034)
Cat. No.: RMMS-00560034
Category: Specialized Medium Kits
Research Area: Stem Cell Biology
INQUIRY
1 Kit
Serum-free supplement and attachment substrate for the derivation and expansion of human skeletal muscle progenitor cells (myoblasts)
Product Features
| Cell Type | Myogenic Stem and Progenitor Cells |
|---|---|
| Formulation | Serum-Free |
| Species | Human |
| Applications | Cell Culture, Expansion |
| Description | Culture primary human skeletal muscle progenitor cells and human pluripotent stem cell (hPSC)-derived myoblasts under serum-free conditions. The -SF Expansion Supplement Kit (Human) includes components optimized for the derivation and expansion of myoblasts in vitro: -SF Expansion 10X Supplement (Human) and -SF Attachment Substrate. The supplement must be combined with a basal medium (DMEM with 1000 mg/L D-Glucose; sold separately) to prepare -SF Expansion Medium. Use of the -SF Attachment Substrate is optional depending on the cell source used. For your convenience, the kit components may also be purchased individually. If you are using primary myoblasts, we recommend coating cultureware with -SF Attachment Substrate. For hPSC-derived myoblasts, you can use hESC-Qualified Matrix. Myoblasts cultured using -SF Expansion Medium are compatible with Differentiation Kit (Human) and can be used for further differentiation into multinucleated myotubes. |
Storage & Shipping
| Storage | -20°C or colder |
|---|---|
| Shipping | Shipped on gel packs. |
For research use only. Not for clinical use.
