Transepithelial/endothelial Electrical Resistance configuration BBB blood brain barrier model chip (RMBC-00230003)
Cat. No.: RMBC-00230003
Category: Bio-Chip
BBB Blood Brain Barrier Model Chip - TEER configuration - Barrier type: Slits, Slit width: 3 μm, Distance between slits: 50 μm, Travel/barrier width: 50 μm, Outer Channel width: 200 μm, Device depth: 100 μm, Impedence measurement. This TEER enabled chip is used to create the BBB model in monoculture, co or tri-culture configurations.
BBB 3D blood brain barrier model recreates the in vivo microenvironment by emulating a histological slice of brain tissue cells in communication with endothelial cells across the blood brain barrier (BBB). Shear-induced endothelial cell tight junctions, which cannot be achieved in the Transwell model, are easily achieved in the BBB model using physiological fluid flow. Formation of tight changes can be measured using biochemical or electrical analysis (assessing changes in electrical resistance) with the cell impedance analyzer. Interactions between brain tissue cells and endothelial cells are readily visualized in the BBB assay. Transwell models do not allow real-time visualization of these cellular interactions, which are critical for understanding of the BBB microenvironment.
This system allows:
Accurate in vivo hemodynamic shear stress
Real-time visualization of cellular and barrier functionality
Significant reduction in cost and time
Robust and easy to use protocols
BBB 3D blood brain barrier model recreates the in vivo microenvironment by emulating a histological slice of brain tissue cells in communication with endothelial cells across the blood brain barrier (BBB). Shear-induced endothelial cell tight junctions, which cannot be achieved in the Transwell model, are easily achieved in the BBB model using physiological fluid flow. Formation of tight changes can be measured using biochemical or electrical analysis (assessing changes in electrical resistance) with the cell impedance analyzer. Interactions between brain tissue cells and endothelial cells are readily visualized in the BBB assay. Transwell models do not allow real-time visualization of these cellular interactions, which are critical for understanding of the BBB microenvironment.
This system allows:
Accurate in vivo hemodynamic shear stress
Real-time visualization of cellular and barrier functionality
Significant reduction in cost and time
Robust and easy to use protocols
Product Features
| Application | The system is a highly versatile platform for investigation of: Tight junction proteins: Determine the levels of tight junction proteins namely zonula occludens, claudins and occludins which regulate the BBB. Transporter proteins: Analyze functionality of transporter proteins in normal and dysfunctional BBB. Drug permeability: Evaluate real-time permeability of therapeutics and small molecules across the endotheliμm of the BBB. Inflammation: Understand the underlying mechanisms of inflammatory responses on the regulation of the BBB. Cell migration: Visualize and quantify in real-time migration of immune cells across the BBB. Omic changes: Perform genomic, proteomic and metabolic analysis on normal and dysfunctional BBB. Neurotoxicity: Analyze toxicity effects of chemical, biological and physical agents on the cells of the BBB. Neuro-oncology: Investigate effects of the tμmor cells on the BBB. |
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| Packaging | Case of 1 ea |
Storage & Shipping
| Storage Temp | Room temperature |
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For research use only. Not for clinical use.
