Alveolar Organoid Medium (RMMS-00560315)
Cat. No.: RMMS-00560315
Category: Organoid Growth
Research Area: Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Infectious Diseases, Organoids, Respiratory Research
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1 Kit
Cell culture media for expansion and differentiation of human alveolar organoids
Product Features
| Overview | Grow, expand, and differentiate organoids from human alveolar epithelial cells. Unlike traditionally used animal models, alveolar organoids mimic the regional specificity of the in vivo alveoli, making them ideally suited for alveolar biology research, infectious disease studies, and drug screening. With the convenience of Alveolar Organoid Medium, you can efficiently passage and expand human alveolar epithelial type II (ATII) cells long term as organoids, then further differentiate these ATII organoids to alveolar epithelial type I (ATI) cells for downstream applications. Use Alveolar Organoid Expansion (AvOE) Medium to achieve a 10,000X cumulative-fold expansion of ATII cells as organoids within 10 passages; this high yield reduces the need to regularly source donor tissue. You can flexibly pause your cultures at any passage by cryopreserving organoids cultured in AvOE Medium as single cells. The medium’s three-component, serum-free format maximizes experimental reproducibility, and the alveolar organoids maintain properties indicative of the ATII cell phenotype, including the ability for self-renewal, expected marker expression (e. g. HT2-280 and SP-C), and lineage potential for differentiation to ATI cells. AvOE Medium can also be used to generate alveolar monolayers to facilitate air-liquid interface (ALI) culture as outlined in this protocol. Use Alveolar Organoid Differentiation (AvOD) Medium to differentiate ATII cells expanded as organoids in AvOE Medium. The two-component AvOD Medium enables in-dome differentiation of ATII organoid cultures to ATI cells in as few as 10 days, with over 85% purity. Differentiated cells show decreased ATII marker expression and strong up-regulation of ATI cell markers (RAGE/AGER, HT1-56, and GPRC5a). AvOE and AvOD Medium are compatible with primary isolated fresh or cryopreserved ATII cells, as well as high-quality commercially available alveolar epithelial cell sources. |
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| Species | Human |
| Cell Type | Airway Cells, Epithelial Cells |
| Applications | Cell Culture, Differentiation, Expansion, Maintenance, Organoid Culture |
Storage & Shipping
| Storage | -20°C or colder |
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| Shipping | Shipped on gel packs. |
For research use only. Not for clinical use.
