Anti-CD59 Antibody (RMAB-0250389)
Cat. No.: RMAB-0250389
Category: Primary Antibodies
INQUIRY
100 μg
Customer Size
Mouse monoclonal to CD59
Product Features
| Isotype | IgG2b |
|---|---|
| Clonality | Monoclonal |
| Host Species | Mouse |
| Clone Number | MEM-43/5 |
| Form | Liquid |
| Purity | Protein A purified |
| Species Reactivity | Human, Mouse |
| Immunogen | Tissue, cells or virus corresponding to Human CD59. Thymocytes and T lymphocytes |
| Applications | ICC/IF, IP, IHC-P, Flow Cyt, WB |
| Key Features | Mouse monoclonal to CD59; Suitable for: ICC/IF, IP, IHC-P, Flow Cyt, WB; Reacts with: Mouse, Human; Isotype: IgG2b |
Target Information
| Target Symbol | CD59 |
|---|---|
| Target Name | CD59 glycoprotein |
| UniProt ID | P13987 |
| Cellular Localization | Cell membrane. Secreted. Soluble form found in a number of tissues. |
| Epitope | The antibody MEM-43/5 reacts with well defined epitope around L33 (see Bodian et al) |
| Function | Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. |
| Involvement in Disease | Defects in CD59 are the cause of CD59 deficiency (CD59D). |
| Post-translational Modifications | N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants. Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. |
| Myeloma | unknown |
| Sequence Similarities | Contains 1 UPAR/Ly6 domain. |
Storage & Shipping
| Storage Buffer | pH: 7.40; Preservative: 0.097% Sodium azide; Constituent: PBS |
|---|---|
| Storage & Shipping | Shipped at 4°C. Store at 4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
For research use only. Not for clinical use.
