Anti-EHMT2/G9A Antibody - ChIP Grade (RMAB-0250540)
Cat. No.: RMAB-0250540
Category: Primary Antibodies
INQUIRY
100 μL
Customer Size
Rabbit monoclonal to EHMT2/G9A - ChIP Grade
Product Features
| Isotype | IgG |
|---|---|
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone Number | EPR4019(2) |
| Form | Liquid |
| Purity | Protein A purified |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide within Human EHMT2/G9A aa 115 to the C-terminus (C terminal). The exact sequence is proprietary. |
| Applications | WB, IHC-P, ChIP-seq |
| Key Features | Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply; Rabbit monoclonal to EHMT2/G9A - ChIP Grade; Suitable for: WB, IHC-P, ChIP-sequencing; Knockout validated; Reacts with: Human |
Target Information
| Target Symbol | EHMT2 |
|---|---|
| Target Name | Histone-lysine N-methyltransferase EHMT2 |
| UniProt ID | Q96KQ7 |
| Cellular Localization | Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin. |
| Function | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. |
| Post-translational Modifications | Methylated at Lys-185; automethylated. |
| Domain | The SET domain mediates interaction with WIZ.The ANK repeats bind H3K9me1 and H3K9me2. |
| Sequence Similarities | Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily. Contains 7 ANK repeats. Contains 1 post-SET domain. Contains 1 pre-SET domain. Contains 1 SET domain. |
Storage & Shipping
| Storage Buffer | pH: 7.2; Preservative: 0.05% Sodium azide; Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant |
|---|---|
| Storage & Shipping | Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. |
For research use only. Not for clinical use.
