Anti-LEF1 Antibody (RMAB-0250812)
Cat. No.: RMAB-0250812
Category: Primary Antibodies
INQUIRY
10 μL
100 μL
Rabbit monoclonal to LEF1
Product Features
| Isotype | IgG |
|---|---|
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone Number | EPR2029Y |
| Form | Liquid |
| Purity | Protein A purified |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide within Human LEF1 aa 1-2. The exact sequence is proprietary. |
| Applications | IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) |
| Key Features | Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply; Rabbit monoclonal to LEF1; Suitable for: IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra); Knockout validated; Reacts with: Mouse, Rat, Human |
Target Information
| Target Symbol | LEF1 |
|---|---|
| Target Name | Lymphoid enhancer-binding factor 1 |
| UniProt ID | Q9UJU2 |
| Cellular Localization | Nucleus. Found in nuclear bodies upon PIASG binding. |
| Function | Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP3. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. |
| Domain | Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors. |
| Sequence Similarities | Belongs to the TCF/LEF family. Contains 1 HMG box DNA-binding domain. |
Storage & Shipping
| Storage Buffer | Preservative: 0.01% Sodium azide; Constituents: 40% Glycerol, 0.05% BSA, PBS |
|---|---|
| Storage & Shipping | Shipped at 4°C. Store at 4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. |
For research use only. Not for clinical use.
