Anti-PP2A alpha + beta Antibody (RMAB-0250989)
Cat. No.: RMAB-0250989
Category: Primary Antibodies
INQUIRY
100 μL
Customer Size
Rabbit monoclonal to PP2A alpha + beta
Product Features
| Isotype | IgG |
|---|---|
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Clone Number | YE351 |
| Form | Liquid |
| Purity | Protein A purified |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide within Human PP2A alpha + beta (N terminal). The exact sequence is proprietary. |
| Applications | IP, WB, IHC-P, Dot blot |
| Key Features | Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply; Rabbit monoclonal to PP2A alpha + beta; Suitable for: IP, WB, IHC-P, Dot blot; Reacts with: Mouse, Rat, Human |
Target Information
| Target Symbol | PPP2CA |
|---|---|
| Target Name | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform |
| UniProt ID | P67775 |
| Cellular Localization | Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2. |
| Epitope | This antibody reacts with an epitope located in the N terminal region of PP2A alpha. |
| Function | PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV4 large T antigen and p53/TP53. Dephosphorylates SV4 large T antigen, preferentially on serine residues 12, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'. |
| Post-translational Modifications | Reversibly methyl esterified on Leu-39. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle. Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. |
| Sequence Similarities | Belongs to the PPP phosphatase family. PP-1 subfamily. |
Storage & Shipping
| Storage Buffer | pH: 7.20; Preservative: 0.01% Sodium azide; Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA |
|---|---|
| Storage & Shipping | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
For research use only. Not for clinical use.
