Anti-Smad2 (phospho T8) + Smad3 (phospho T8) Antibody

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Anti-Smad2 (phospho T8) + Smad3 (phospho T8) Antibody (RMAB-0251047)

Cat. No.: RMAB-0251047

Category: Primary Antibodies

INQUIRY 100 μL Customer Size

Rabbit monoclonal to Smad2 (phospho T8) + Smad3 (phospho T8)

Product Features

Isotype	IgG
Clonality	Monoclonal
Host Species	Rabbit
Clone Number	EPR23682-64
Form	Liquid
Purity	Protein A purified
Species Reactivity	Human, Mouse, Rat
Immunogen	This product was produced with the following immunogens:Synthetic peptide.Synthetic peptide.
Applications	ICC/IF, Dot blot, IP, ChIC/CUT&RUN-seq, WB
Key Features	Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply; Rabbit monoclonal to Smad2 (phospho T8) + Smad3 (phospho T8); Suitable for: ICC/IF, Dot blot, IP, ChIC/CUT&RUN-seq, WB; Reacts with: Mouse, Rat, Human

Target Information

Target Name	Smad2 (phospho T8) + Smad3 (phospho T8)
Cellular Localization	Smad2: Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Smad3: Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236).

Target Name

Smad2 (phospho T8) + Smad3 (phospho T8)

Cellular Localization

Smad2: Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.
Smad3: Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236).

Storage & Shipping

Storage Buffer	Preservative: 0.01% Sodium azide; Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage & Shipping	Shipped at 4°C. Store at 4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

For research use only. Not for clinical use.