Anti-Vimentin Antibody - Cytoskeleton Marker (RMAB-0251187)
Cat. No.: RMAB-0251187
Category: Primary Antibodies
INQUIRY
100 μg
Customer Size
Rabbit polyclonal to Vimentin - Cytoskeleton Marker
Product Features
| Isotype | IgG |
|---|---|
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Form | Liquid |
| Purity | Immunogen affinity purified |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide conjugated to KLH derived from within residues 4 to the C-terminus of Human Vimentin. Read We's proprietary immunogen policy |
| Applications | IHC-P, WB, ICC/IF, Flow Cyt (Intra) |
| Key Features | Rabbit polyclonal to Vimentin - Cytoskeleton Marker; Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra); Knockout validated; Reacts with: Mouse, Rat, Human; Isotype: IgG |
Target Information
| Target Symbol | VIM |
|---|---|
| Target Name | Vimentin |
| UniProt ID | P08670 |
| Cellular Localization | Cytoplasm. |
| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. |
| Involvement in Disease | Cataract 3 |
| Post-translational Modifications | Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S1A8/9 transnitrosylase complex. |
| Domain | The central alpha-helical coiled-coil rod region mediates elementary homodimerization.The-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S1A8/A9 transnitrosylase complex. |
| Sequence Similarities | Belongs to the intermediate filament family. |
Storage & Shipping
| Storage Buffer | pH: 7.40; Preservative: 0.02% Sodium azide; Constituent: PBSBatches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. |
|---|---|
| Storage & Shipping | Shipped at 4°C. Store at 4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
For research use only. Not for clinical use.
