ASC52telo, hTERT immortalized adipose derived Mesenchymal stem cells (RMCL-00260282)
Cat. No.: RMCL-00260282
Category: hTERT-immortalized cell
INQUIRY
1 vial
ASC52telo is an hTERT immortalized adipose derived Mesenchymal stem cell line exhibiting a fibroblast-like morphology that was isolated in 2006 from the adipose tissue of a White female. The cell line can be used in stem cell research.
Product Features
| BSL | BSL-2 |
|---|---|
| Product Format | Frozen |
| Applications | Stem cell research |
| Product Type | Human cells |
| Organism | Homo sapiens, human |
| Tissue | Adipose tissue |
| Morphology | Fibroblast-like |
| Cell Type | Mesenchymal Stem Cell |
| STR Profiling | Amelogenin: XCSF1PO: 10, 13D13S317: 8, 12D16S539: 10, 13D5S818: 11, 13D7S820: 8, 11THO1: 7TPOX: 8vWA: 16 |
| Antigen Expression | Positive surface markers: CD29, CD44, CD73, CD90, CD105, CD166 (>90%)Negative surface markers: CD14, CD19, CD34, CD45 (<5%) |
| Genes Expressed | CD29+ ; CD44+; CD73+; CD90+ ; CD105+; CD166+ |
QC
| Mycoplasma Contamination | Not detected |
|---|
Doner Information
| Gender | Female |
|---|---|
| Ethnicity | White |
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Reagents for Cryopreservation | 90% Complete growth media; DMSO, 10% |
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Growth Properties | Adherent |
| Temperature | 37°C |
| Atmosphere | 95% Air, 5% CO2 |
| Handling procedure | Recovery of Frozen Cells To ensure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If storage of the frozen culture is necessary upon arrival, store the vial in liquid nitrogen vapor phase and NOT at -70°C. Storage at -70°C will result in loss of viability. SAFETY PRECAUTION: Always use protective gloves and clothing and wear a full face mask when handling frozen vials. Some vials leak when immersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vessel exploding or blowing off its cap with dangerous force creating flying debris. 1. Prepare a 25-cm2 or a 75-cm2 culture flask containing the recommended complete culture medium. Prior to the addition of the vial contents, the vessel containing the growth medium should be placed in the incubator for at least 15 minutes to allow the medium to reach its normal pH (7. 0 to 7. 6) and to avoid excessive alkalinity of the medium during recovery of the cells. 2. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes). 3. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All operations from this point on should be carried out under strict aseptic conditions. 4. Transfer the vial contents to a centrifuge tube containing 9. 0 ml of complete culture medium and centrifuge the cell suspension at approximately 275 x g +/- 125 x g for 5 to 7 minutes. 5. Discard the supernatant and resuspend the cells in fresh growth medium. Count the cells and seed new culture flasks at a density of 5,000 viable cells per cm2. 6. Incubate the culture at 37°C in a suitable incubator. 7. A 5% CO2/95% air atmosphere is recommended if using the medium described on this product sheet. |
| Population Doubling Time | Approximately 45 hrs |
| Population Doubling Capacity | ≥ 25 in complete growth medium |
For research use only. Not for clinical use.
