CHO CD Medium (RMMS-00560269)
Cat. No.: RMMS-00560269
Category: Specialized Medium
Research Area: Antibody Development, Cell Line Development, Drug Discovery and Toxicity Testing
INQUIRY
90 mL
Chemically defined, animal component-free, serum-free, protein-free, glutamine-free semi-solid methylcellulose-based medium for selecting and cloning CHO cells
Product Features
| Overview | This product is a methylcellulose-based semi-solid medium recommended for selection and cloning of Chinese hamster ovary (CHO) cells. This medium is chemically defined, protein-free, and animal component-free. It does not contain L-glutamine, selection agents, or phenol red and is compatible with dihydrofolate reductase (DHFR) and glutamine synthetase (GS) selection systems. Benefits of semi-solid cloning: Individual clones and their progeny remain localized together in a semi-solid matrix as they grow to form distinct monoclonal colonies. This prevents the loss of rare, high-producing clones by overgrowth from faster-growing cells, as can occur during selection in a liquid medium, and facilitates the isolation of a diverse set of clones with a wide range of growth rates and productivities to be obtained for downstream screening. Colonies obtained from semi-solid medium have a high probability of monoclonality, allowing clonal cell lines to be generated in less time and using fewer resources than with limiting dilution cloning. Colonies can be easily picked from the semi-solid medium by manual or robotic methods and dispersed into a liquid growth medium for screening and expansion. |
|---|---|
| Species | Other |
| Cell Type | CHO Cells |
| Applications | Cell Culture, Semi-Solid Cloning |
| Formulation | Animal Component-Free, Chemically Defined, Protein-Free, Serum-Free |
Storage & Shipping
| Storage | -20°C or colder |
|---|---|
| Shipping | Shipped on gel packs. |
For research use only. Not for clinical use.
