Heat Stable Recombinant Human FGF-basic (154 a.a.) (RMPP-00231181)
Cat. No.: RMPP-00231181
Category: Growth Factors & Cytokines
INQUIRY
10 μg
50 μg
FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic exerts proliferative activity towards a wide range of cell types and consequently is used as a vital component in numerous cell culture media formulations, including stem cell media. FGF-basic loses its activity over time in solution at physiological temperatures and needs to be resupplemented periodically to maintain its performance. As a result of selected amino acid substitutions, Heat Stable FGF-basic retains full biological activity and also maintains full activity over extended periods of time in solution at 37°C. These properties make Heat Stable FGF-basic a superior choice for use in cell culture media applications. Heat Stable Recombinant Human FGF-basic is a 17.2 kDa protein consisting of 154 amino acids.Heat Stable Recombinant Human FGF-basic (154 a.a.) was developed under a license from Enantis.
Product Features
| Source | E.coli |
|---|---|
| Purity | ≥ 95% by SDS-PAGE gel and HPLC analyses. |
| Nature | Recombinant |
| Endotoxin Level | < 1 Eu/μg |
Protein Information
| UniProt ID | P09038 |
|---|---|
| Molecular Weight | 17.2 kDa |
| Sequence Similarities | Belongs to the heparin-binding growth factors family. |
| Protein Length | Full length protein |
| Cellular Localization | Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. |
| Tissue Specificity | Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. |
| Function | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis. |
| Post-translational Modifications | Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. |
Storage & Shipping
| Shipping and Storage | Shipped on Dry Ice. |
|---|
For research use only. Not for clinical use.
