hESC BG01V (RMCL-00260249)
Cat. No.: RMCL-00260249
Category: Cell Lines
INQUIRY
1 vial
hESC BG01V is a fibroblast cell that was isolated from the inner cell mass of an embryo. hESC BG01V cells are pluripotent and can differentiate into representatives of the three primary germ layers.
Product Features
| BSL | BSL-1 |
|---|---|
| Product Format | Frozen |
| Applications | Stem cell research |
| Specific Applications | This product is pluripotent and can differentiate to representatives of the three primary germ layers. |
| Product Type | Human cells |
| Organism | Homo sapiens, human |
| Tissue | Embryo; Inner cell mass |
| Morphology | Spherical colony |
| Cell Type | Embryonic Stem Cell |
| STR Profiling | Amelogenin: X,Y CSF1PO: 10 D13S317: 11,12 D16S539: 9,11 D5S818: 10,12 D7S820: 10,11 TH01: 7,9.3 TPOX: 8 vWA: 16,17 D3S1358: 15,17 D21S11: 28,29 D18S51: 19 Penta_E: 13,14 Penta_D: 12 D8S1179: 10,12 FGA: 22 D19S433: 13,15 D2S1338: 17,24 |
| Karyotype | 49, XXY, +12, +17 |
| Antigen Expression | The cells stain positive for pluripotency markers and alkaline phosphatase activity. |
QC
| Mycoplasma Contamination | Not detected |
|---|
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Temperature | 37°C |
| Atmosphere | 95% Air, 5% CO2 |
| Handling procedure | To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If, upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at –80°C. Storage at –80°C will result in loss of viability. Preparation for Culture Night before thawing iPSC cells - Thaw Cell Basement Membrane on ice in refrigerator or cold room (2°C to 8°C) One Hour Prior to Thawing the iPS Cells - Prepare coated plates as described. 30 Minutes Prior to Handling Cells - Pre-warm Pluripotent Stem Cell SFM XF/FF (stem cell culture medium) at 37°C for at least 30 minutes before adding to cells. If using ROCK Inhibitor Y27632, prepare stem cell culture medium supplemented with final concentration of 10 μM ROCK Inhibitor Y27632. Stem cell culture medium with ROCK inhibitor must be used immediately. |
For research use only. Not for clinical use.
