Human BMI1 ELISA Kit (RMEK-0152399)
Cat. No.: RMEK-0152399
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human BMI1 ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BMI1 protein in cell and tissue extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-1.3% |
| Sensitivity | 34 pg/mL |
| Range | 78.13 pg/mL - 5000 pg/mL |
| Sample Type | Cell Lysate |
| Recovery | Cell Lysate-114-112% - 115% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 34 pg/mL; Range: 78.13 pg/mL - 5000 pg/mL; Sample type: Cell Lysate; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | BMI1 |
|---|---|
| UniProt ID | P35226 |
| Biomarker of SCs/CSCs | Pancreatic Cancer |
| Function | Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. |
| Cellular Localization | Nucleus. Cytoplasm. |
| Post-transcriptional Modifications | Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
