Human LEF1 ELISA Kit (RMEK-0152773)
Cat. No.: RMEK-0152773
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human LEF1 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human LEF1 in cell extract samples. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-2.4%; Inter-Assay-Extract-4-5.4% |
| Sensitivity | 4.573 pg/mL |
| Range | 9.375 pg/mL - 600 pg/mL |
| Sample Type | Cell Lysate |
| Recovery | Cell Lysate-91-86% - 100% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 4.573 pg/mL; Range: 9.375 pg/mL - 600 pg/mL; Sample type: Cell Lysate; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | LEF1 |
|---|---|
| UniProt ID | Q9UJU2 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. |
| Cellular Localization | Nucleus. Found in nuclear bodies upon PIASG binding. |
| Domain | Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
