Human CD105 ELISA Kit (RMEK-0152455)
Cat. No.: RMEK-0152455
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human CD105 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD105 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Human Serum-3-1.8%; Inter-Assay-Human Serum-5-3% |
| Sensitivity | 11.1 pg/mL |
| Range | 78.125 pg/mL - 5000 pg/mL |
| Sample Type | Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts |
| Recovery | Serum-107-84% - 120%; Cell culture extracts-103-99% - 110%; Tissue Extracts-106-96% - 119%; Cell culture media-107-95% - 116%; Hep Plasma-88-71% - 113%; EDTA Plasma-100-84% - 119%; Cit plasma-80-74% - 84% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 11.1 pg/mL; Range: 78.125 pg/mL - 5000 pg/mL; Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | CD105 |
|---|---|
| UniProt ID | P17813 |
| Biomarker of SCs/CSCs | Multipotent Adult Stem Cells Characterization |
| Function | Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. |
| Cellular Localization | Membrane. |
| Involvement in Disease | Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1); also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
