Human CDCP1 ELISA Kit (RMEK-0152490)
Cat. No.: RMEK-0152490
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human CDCP1 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CDCP1 protein in tissue extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-24-3.2%; Inter-Assay-Extract-3-3.2% |
| Sensitivity | 221 ng/mL |
| Range | 0.31 ng/mL - 20 ng/mL |
| Sample Type | Tissue Extracts |
| Recovery | Tissue Extracts-106-100% - 109% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 221 ng/mL; Range: 0.31 ng/mL - 20 ng/mL; Sample type: Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | CDCP1 |
|---|---|
| UniProt ID | Q9H5V8 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis. |
| Cellular Localization | Secreted and Cell membrane. Shedding may also lead to a soluble peptide. |
| Post-transcriptional Modifications | Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.N-glycosylated.A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
