Human Colon Cells (RMCL-00260130)
Cat. No.: RMCL-00260130
Category: Cell model; Organoid
INQUIRY
1 vial
A patient-derived next-generation cancer model. This cell line was isolated from primary colon adenocarcinoma tissue. This tumor-derived model can be used in basic research and pharmacological screening applications.
Product Features
| BSL | BSL-1 |
|---|---|
| Product Format | Frozen |
| Applications | 3D cell culture; Cancer research |
| Specific Applications | Basic research, compound screening. |
| Product Type | Human cells |
| Organism | Homo sapiens, human |
| Tissue | Large intestine; Colon |
| Morphology | Organoid |
| Disease | Adenocarcinoma; Primary |
QC
| Bacterial & Fungal Testing | Not detected |
|---|---|
| Mycoplasma Contamination | Not detected |
| Virus Testing | Cytomegalovirus (CMV): Not detected Epstein-Barr virus (EBV): Not detected Hepatitis B virus (HBV): Not detected Human Immunodeficiency virus (HIV): Not detected Human papillomavirus (HPV): Not detected |
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Reagents for Cryopreservation | We recommend cryopreserving this model in Stem Cell Freezing Media. |
| Growth Properties | Embedded 3D culture |
| Temperature | 37°C |
| Atmosphere | 95% Air, 5% CO2 |
| Handling procedure | Seeding density: We recommend seeding this model at 0. 25 - 1 x 106/ viable cells in 100 µL of ECM per well of a 6-well plate. ECM: We recommend culturing this model in Cell Basement Membrane or Corning Matrigel. Include 10 µM ROCK Inhibitor Y-27632 in medium for the first 2-3 days following subculture. For a brief overview of the thawing procedure see our quickstart guide Thawing Cryopreserved Human Organoids. |
For research use only. Not for clinical use.
