Human FABP2 ELISA Kit (RMEK-0152591)
Cat. No.: RMEK-0152591
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human FABP2 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FABP2 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-9-1.8%; Inter-Assay-Serum-3-3.3% |
| Sensitivity | 1.7 pg/mL |
| Range | 12.5 pg/mL - 800 pg/mL |
| Sample Type | Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine |
| Recovery | Urine-83-82% - 85%; Serum-119-111% - 125%; Cell culture extracts-95-92% - 97%; Cell culture media-100-98% - 102%; Hep Plasma-108-103% - 110%; EDTA Plasma-115-109% - 120%; Cit plasma-113-105% - 118% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 1.7 pg/mL; Range: 12.5 pg/mL - 800 pg/mL; Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | FABP2 |
|---|---|
| UniProt ID | P12104 |
| Function | FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. |
| Cellular Localization | Cytoplasm. |
| Domain | Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
