Human FGF19 ELISA Kit (RMEK-0152602)
Cat. No.: RMEK-0152602
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human FGF19 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGF19 protein in cit plasma, edta plasma, hep plasma, and serum. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Supernatant-5-3.8%; Inter-Assay-Supernatant-3-6.6% |
| Sensitivity | 1.4 pg/mL |
| Range | 3.91 pg/mL - 250 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Serum-112-106% - 117%; Cell culture media-108-107% - 110%; Hep Plasma-108-101% - 116%; EDTA Plasma--94% - 99%; Cit plasma-100-97% - 105% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 1.4 pg/mL; Range: 3.91 pg/mL - 250 pg/mL; Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | FGF19 |
|---|---|
| UniProt ID | O95750 |
| Function | Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB. |
| Cellular Localization | Secreted. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
