Human FOXA2 ELISA Kit (RMEK-0152626)
Cat. No.: RMEK-0152626
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human FOXA2 kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FOXA2 protein in human cell extract. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-4.4%; Inter-Assay-Extract-3-4.3% |
| Sensitivity | 34.643 pg/mL |
| Range | 125 pg/mL - 8000 pg/mL |
| Sample Type | Cell Lysate |
| Recovery | Cell Lysate-87-83% - 89% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 34.643 pg/mL; Range: 125 pg/mL - 8000 pg/mL; Sample type: Cell Lysate; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | FOXA2 |
|---|---|
| UniProt ID | Q9Y261 |
| Biomarker of SCs/CSCs | Hepatic Cancer |
| Function | Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation. |
| Cellular Localization | Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner and in response to insulin signaling via AKT1 is exported from the nucleus. |
| Post-transcriptional Modifications | Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
