Human Osteopontin ELISA Kit (RMEK-0152826)
Cat. No.: RMEK-0152826
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human Osteopontin ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Osteopontin protein in edta plasma, hep plasma, milk, serum, urine, cit plasma, and cell culture supernatant. The technology. Quantitate Human Osteopontin with 88 pg/mL sensitivity. _x000D_
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-3-2.1%; Inter-Assay-Serum-8-2.8% |
| Sensitivity | 88 pg/mL |
| Range | 109.38 pg/mL - 7000 pg/mL |
| Sample Type | Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Serum, Urine |
| Recovery | Cell culture supernatant-108-102% - 115%; Milk-96-93% - 98%; Urine-98-96% - 100%; Serum-102-99% - 104%; Hep Plasma-93-90% - 96%; EDTA Plasma-99-95% - 102%; Cit plasma-92-91% - 93%; Cerebral Spinal Fluid-86-82% - 93% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 88 pg/mL; Range: 109.38 pg/mL - 7000 pg/mL; Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Serum, Urine; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | SPP1 |
|---|---|
| UniProt ID | P10451 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Cellular Localization | Secreted. |
| Post-transcriptional Modifications | Extensively phosphorylated on clustered serine residues.N- and O-glycosylated.Phosphorylation sites are present in the extracelllular medium. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
