Human S100B ELISA Kit- Extracellular (RMEK-0152868)
Cat. No.: RMEK-0152868
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human S100B kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of S100B protein in Serum, Milk, Cerebral Spinal Fluid. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Milk-8-8%; Inter-Assay-Milk-3-5.2% |
| Sensitivity | 1.469 pg/mL |
| Range | 12.5 pg/mL - 800 pg/mL |
| Sample Type | Cerebral Spinal Fluid, Milk, Serum |
| Recovery | Milk-90-87% - 95%; Serum-85-76% - 91%; Cerebral Spinal Fluid-107-98% - 114% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 1.469 pg/mL; Range: 12.5 pg/mL - 800 pg/mL; Sample type: Cerebral Spinal Fluid, Milk, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | S100B |
|---|---|
| UniProt ID | P04271 |
| Biomarker of SCs/CSCs | Colon Cancer |
| Function | Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. |
| Cellular Localization | Cytoplasm. Nucleus. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
