Human TGFB3 ELISA Kit (RMEK-0152956)
Cat. No.: RMEK-0152956
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human TGFB3 ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TGFB3 protein in cell culture supernatant and cell and tissue extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-5.2%; Inter-Assay-Extract-3-11.1% |
| Sensitivity | 0.038 ng/mL |
| Range | 0.38 ng/mL - 24 ng/mL |
| Sample Type | Cell culture media, Tissue Extracts |
| Recovery | Tissue Extracts-91-86% - 96%; Cell culture media-100-89% - 107% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 0.038 ng/mL; Range: 0.38 ng/mL - 24 ng/mL; Sample type: Cell culture media, Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | TGFB3 |
|---|---|
| UniProt ID | P10600 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Involved in embryogenesis and cell differentiation. |
| Cellular Localization | Secreted. |
| Domain | The 'straitjacket' and 'arm' domains encircle the Transforming growth factor beta-1 (TGF-beta-1) monomers and are fastened together by strong bonding between Lys-56 and Tyr-103/Tyr-104. The cell attachment site motif mediates binding to integrins (ITGAV:ITGB6 or ITGAV:ITGB8). The motif locates to a long loop in the arm domain called the bowtie tail. Integrin-binding stabilizes an alternative conformation of the bowtie tail. Activation by integrin requires force application by the actin cytoskeleton, which is resisted by the 'milieu molecules' (such as LTBP1, LRRC32/GARP and/or LRRC33/NRROS), resulting in distortion of the prodomain and release of the active TGF-beta-1. |
| Post-transcriptional Modifications | Transforming growth factor beta-3 proprotein: The precursor proprotein is cleaved in the Golgi apparatus to form Transforming growth factor beta-3 (TGF-beta-3) and Latency-associated peptide (LAP) chains, which remain non-covalently linked, rendering TGF-beta-3 inactive. Methylated at Gln-293 by N6AMT1. |
| Involvement in Disease | Defects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1); also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
